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About Rose

Solis BioDyne PCR Enzymes, qPCR Dye and Probe Based Reagents, cDNA Mixes

  Overview

Solis BioDyne has been developing and producing life science reagents since 1995.

Patented technology makes the reagents stable at ambient temperatures for at least one month, so that products can be shipped without ice and reaction set-up does not require ice.

All enzymes produced at Solis BioDyne are exceptionally stable at room temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide shelf life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at room temperature for one month, there is no detectable change in the performance of the enzymes.

Stability TAG Technology is now applied in all of Solis BioDyne's products providing enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at room temperature.

Tests with different Stability TAG attached proteins revealed no effect on protein properties. For example, pluripotency was maintained in ES cells during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as with regular Taq.

Enzymes produced at Solis BioDyne also exhibit extraordinarily long shelf life, measured in decades, if stored at -20°C.

All Solis BioDyne PCR and qPCR mixes are produced in 5x concentrations to improve stability. The higher buffer concentration also leaves 2.5 times more room for template and primers compared with 2x mixes. This is highly advantageous when working with low-concentration DNA samples or performing multiplex assays.

Call Rose Scientific at 780.438.5110 or .800.661.9289, or email sales@rosesci.com for information on availability, quantities and prices!

Features of the HOT FIREPol PCR Enzymes:

HOT FIREPol® DNA Polymerase

  • Increased specificity and sensitivity
  • Reduced primer dimer formation
  • Reaction set-up and shipment without dry ice

5x HOT FIREPol® GC Master Mix
  • Excellent amplification with templates up to 79% GC content
  • Suitable for templates up to 5 kb
  • Vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization
  • Reaction set-up and shipment without ice
5x HOT FIREPol® Multiplex Mix and Multiplex Mix Ready to Load
  • Analyze multiple targets per reaction
  • Increased sensitivity and yield
  • Prevents primer dimer formation
  • Reaction set-up and shipment without ice
5x HOT FIREPol® Blend Master Mix and Blend Master Mix Ready to Load
  • Increased yield, sensitivity and specificity
  • Up to 5x higher fidelity
  • Suitable for templates up to 5 kb
  • Reduced primer dimer formation
  • Reaction set-up and shipment without ice
Call Rose Scientific at 780.438.5110 or .800.661.9289, or email sales@rosesci.com for information on availability, quantities and prices!

  Features of the 5x HOT FIREPol qPCR Mixes, Dye and Probe Based Approach:

5x HOT FIREPol® SolisGreen qPCR Mix
  • Improved detection of low target concentrations
  • Higher fluorescence level
  • Compatible on most cyclers
  • Reaction set-up and shipment without ice
5x HOT FIREPol® EvaGreen® qPCR Supermix
  • High sensitivity with low DNA concentrations
  • One qPCR mix for all cyclers (except capillary)
  • Contains dUTP to prevent cross-contamination with UNG treatment
  • Reduced primer dimer formation
  • Reaction set-up and shipment without ice
5x HOT FIREPol® EvaGreen® qPCR Mix Plus
  • High sensitivity and specificity
  • Excellent efficiency
  • Master mixes for ROX, no ROX and capillary cyclers
  • Reaction set-up and shipment without ice
5x HOT FIREPol® EvaGreen® HRM Mix
  • Mixes available for ROX and no ROX cyclers
  • Sensitive EvaGreen® dye allows detection of DNA sequence variations
  • Reaction set-up and shipment without ice
5x HOT FIREPolR Probe qPCR Mix Plus
  • Suitable for singleplex and multiplex assays
  • High specificity and sensitivity
  • Master mixes for ROX, no ROX and capillary cyclers
  • Reaction set-up and shipment without ice
5x HOT FIREPol® Probe Universal qPCR Mix
  • Suitable for singleplex and multiplex assays
  • High specificity and sensitivity
  • One qPCR mix for all cyclers (except capillary)
  • Superior results with templates with up to 75% GC content
  • Contains dUTP to prevent cross-contamination with UNG treatment
  • Reaction set-up and shipment without ice
Call Rose Scientific at 780.438.5110 or .800.661.9289, or email sales@rosesci.com for information on availability, quantities and prices!

  Features of the cDNA synthesis enzymes:

FIREScript RT cDNA Synthesis Mix
  • Excellent enzyme for standard applications
  • Reaction temperature up to 50°C
  • Full RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice
FIREScript RT cDNA Synthesis Kit
  • Excellent enzyme for standard applications
  • Reaction temperature up to 50°C
  • Full RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice
FIREScript RT Kit
  • Excellent enzyme for standard applications
  • Reaction temperature up to 50°C
  • Full RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice
SOLIScript RT cDNA Synthesis Mix
  • Superior specificity with complex templates and specific primers
  • Reaction temperature up to 60°C
  • Reduced RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice
SOLIScript RT cDNA Synthesis Kit
  • Superior specificity with complex templates and specific primers
  • Reaction temperature up to 60°C
  • Reduced RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice
SOLIScript RT Kit
  • Superior specificity with complex templates and specific primers
  • Reaction temperature up to 60°C
  • Reduced RNASE H Activity
  • Full length cDNA
  • Reaction set-up and shipment without ice

Call Rose Scientific at 780.438.5110 or .800.661.9289, or email sales@rosesci.com for information on availability, quantities and prices!

Specifications (Click here):  Product Selection Guide, PCR Enzymes

Specifications (Click here):  Product Selection Guide, cDNA Synthesis

Specifications (Click here):  Product Selection Guide, qPCR Compatibility by Reagent

Specifications (Click here):  Product Selection Guide, qPCR Compatibility by PCR Platform

NumberDescriptionPrice $
08-46-00001 5x HOT FIREPol® SolisGreen qPCR Mix, 250 rxn/20 µL Inquire
08-46-00008 5x HOT FIREPol® SolisGreen qPCR Mix, 2000 rxn/20 µL Inquire
08-46-00020 5x HOT FIREPol® SolisGreen qPCR Mix, 5000 rxn/20 µL Inquire
08-36-00001 5x HOT FIREPol® EvaGreen® qPCR Supermix 250 rxn/20 µL Inquire
08-36-00008 5x HOT FIREPol® EvaGreen® qPCR Supermix 2000 rxn/20 µL Inquire
08-36-00020 5x HOT FIREPol® EvaGreen® qPCR Supermix 5000 rxn/20 µL Inquire
08-24-00001 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) 250 rxn/20 µL Inquire
08-24-00008 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) 2000 rxn/20 µL Inquire
08-24-00020 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) 5000 rxn/20 µL Inquire
08-25-00001 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (No ROX) 250 rxn/20 µL Inquire
08-25-00008 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (No ROX) 2000 rxn/20 µL Inquire
08-25-00020 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (No ROX) 5000 rxn/20 µL Inquire
08-26-00001 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) 250 rxn/20 µL Inquire
08-26-00008 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) 2000 rxn/20 µL Inquire
08-26-00020 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) 5000 rxn/20 µL Inquire
08-33-00001 5x HOT FIREPol® EvaGreen® HMR Mix (ROX) 250 rxn/20 µL Inquire
08-33-00008 5x HOT FIREPol® EvaGreen® HMR Mix (ROX) 2000 rxn/20 µL Inquire
08-33-00020 5x HOT FIREPol® EvaGreen® HMR Mix (ROX) 5000 rxn/20 µL Inquire
08-31-00001 5x HOT FIREPol® EvaGreen® HMR Mix (no ROX) 250 rxn/20 µL Inquire
08-31-00008 5x HOT FIREPol® EvaGreen® HMR Mix (no ROX) 2000 rxn/20 µL Inquire
08-31-00020 5x HOT FIREPol® EvaGreen® HMR Mix (no ROX) 5000 rxn/20 µL Inquire
08-17-00001 5x HOT FIREPol® Probe Universal qPCR Mix, 250 rxn/20 µL Inquire
08-17-00008 5x HOT FIREPol® Probe Universal qPCR Mix, 2000 rxn/20 µL Inquire
08-17-00020 5x HOT FIREPol® Probe Universal qPCR Mix, 5000 rxn/20 µL Inquire
08-14-00001 5x HOT FIREPol® Probe qPCR Mix Plus (ROX) 250 rxn/20 µL Inquire
08-14-00008 5x HOT FIREPol® Probe qPCR Mix Plus (ROX) 2000 rxn/20 µL Inquire
08-14-00020 5x HOT FIREPol® Probe qPCR Mix Plus (ROX) 5000 rxn/20 µL Inquire
08-15-00001 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) 250 rxn/20 µL Inquire
08-15-00008 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) 2000 rxn/20 µL Inquire
08-15-00020 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) 5000 rxn/20 µL Inquire
08-16-00001 5x HOT FIREPol® Probe qPCR Mix Plus (Capillary) 250 rxn/20 µL Inquire
08-16-00008 5x HOT FIREPol® Probe qPCR Mix Plus (Capillary) 2000 rxn/20 µL Inquire
08-16-00020 5x HOT FIREPol® Probe qPCR Mix Plus (Capillary) 5000 rxn/20 µL Inquire
01-01-00500 FIREPol® DNA Polymerase (5 U/μL), 500 U Inquire
01-01-01000 FIREPol® DNA Polymerase (5 U/μL), 1000 U Inquire
01-01-02000 FIREPol® DNA Polymerase (5 U/μL), 2000 U Inquire
04-11-00115 5x FIREPol® Master Mix with 7,5 mM MgCl2, 250 rxn/20 µL Inquire
04-11-00125 5x FIREPol® Master Mix with 12.5 mM MgCl2, 250 rxn/20 µL Inquire
04-12-00115 5x FIREPol® Master Mix Ready to Load with 7.5 mM MgCl2, 250 rxn/20 µL Inquire
04-12-00125 5x FIREPol® Master Mix Ready to Load with 12.5 mM MgCl2, 250 rxn/20 µL Inquire
01-02-00500 HOT FIREPol® DNA Polymerase (5U/μL), 500 U Inquire
01-02-01000 HOT FIREPol® DNA Polymerase (5U/μL), 1000 U Inquire
04-33-00115 5x HOT FIREPol® GC Master Mix, 250 rxn/20 µL Inquire
04-33-02015 5x HOT FIREPol® GC Master Mix, 5000 rxn/20 µL Inquire
04-34-00120 5x HOT FIREPol® MultiPlex Mix with 10 mM MgCl2, 250 rxn/20 µL Inquire
04-34-02020 5x HOT FIREPol® MultiPlex Mix with 10 mM MgCl2, 5000 rxn/20 µL Inquire
04-36-00120 5x HOT FIREPol® MultiPlex Mix Ready to Load with 10 mM MgCl2, 250 rxn/20 µL Inquire
04-36-02020 5x HOT FIREPol® MultiPlex Mix Ready to Load with 10 mM MgCl2, 5000 rxn/20 µL Inquire
04-27-00115 5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl2, 250 rxn/20 µL Inquire
04-27-02015 5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl2, 5000 rxn/20 µL Inquire
04-27-00120 5x HOT FIREPol® Blend Master Mix with 10 mM MgCl2, 250 rxn/20 µL Inquire
04-27-02020 5x HOT FIREPol® Blend Master Mix with 10 mM MgCl2, 5000 rxn/20 µL Inquire
04-27-00125 5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2, 250 rxn/20 µL Inquire
04-27-02025 5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2, 5000 rxn/20 µL Inquire
04-27-00130 5x HOT FIREPol® Blend Master Mix with 15 mM MgCl2, 250 rxn/20 µL Inquire
04-27-02030 5x HOT FIREPol® Blend Master Mix with 15 mM MgCl2, 5000 rxn/20 µL Inquire
04-25-00115 5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2, 250 rxn/20 µL Inquire
04-25-02015 5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2, 5000 rxn/20 µL Inquire
04-25-00120 5x HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl2, 250 rxn/20 µL Inquire
04-25-02020 5x HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl2, 5000 rxn/20 µL Inquire
04-25-00125 5x HOT FIREPol® Blend Master Mix Ready to Load with 12.5 mM MgCl2, 250 rxn/20 µL Inquire
04-25-02025 5x HOT FIREPol® Blend Master Mix Ready to Load with 12.5 mM MgCl2, 5000 rxn/20 µL Inquire
04-25-00130 5x HOT FIREPol® Blend Master Mix Ready to Load with 15 mM MgCl2, 250 rxn/20 µL Inquire
04-25-02030 5x HOT FIREPol® Blend Master Mix Ready to Load with 15 mM MgCl2, 5000 rxn/20 µL Inquire
06-20-00100 FIREScript RT cDNA synthesis MIX with Oligo (dT) and Random primers, 100 rxn/20 µL Inquire
06-20-00500 FIREScript RT cDNA synthesis MIX with Oligo (dT) and Random primers, 500 rxn/20 µL Inquire
06-18-00100 FIREScript RT cDNA synthesis MIX with Oligo (dT) primer, 100 rxn/20 µL Inquire
06-18-00500 FIREScript RT cDNA synthesis MIX with Oligo (dT) primer, 500 rxn/20 µL Inquire
06-19-00100 FIREScript RT cDNA synthesis MIX with Random primers, 100 rxn/20 µL Inquire
06-19-00500 FIREScript RT cDNA synthesis MIX with Random primers, 500 rxn/20 µL Inquire
06-17-00100 FIREScript RT cDNA synthesis MIX without primers, 100 rxn/20 µL Inquire
06-17-00500 FIREScript RT cDNA synthesis MIX without primers, 500 rxn/20 µL Inquire
06-15-00050 FIREScript RT cDNA synthesis KIT, 50 rxn/20 µL Inquire
06-15-00200 FIREScript RT cDNA synthesis KIT, 200 rxn/20 µL Inquire
06-13-00050 FIREScript KIT, 50 rxn/20 µL Inquire
06-13-00200 FIREScript KIT, 200 rxn/20 µL Inquire
06-37-00100 SOLIScript RT cDNA synthesis MIX, 100 rxn/20 µL Inquire
06-37-00500 SOLIScript RT cDNA synthesis MIX, 500 rxn/20 µL Inquire
06-35-00050 SOLIScript RT cDNA synthesis KIT, 50 rxn/20 µL Inquire
06-35-00200 SOLIScript RT cDNA synthesis KIT, 200 rxn/20 µL Inquire
06-33-00050 SOLIScript KIT, 50 rxn/20 µL Inquire
06-33-00100 SOLIScript KIT, 200 rxn/20 µL Inquire
06-22-00400 RiboGrip RNase Inhibitor, 20 rxn/20 µL Inquire
06-22-01000 RiboGrip RNase Inhibitor, 50 rxn/20 µL Inquire
06-22-04000 RiboGrip RNase Inhibitor, 200 rxn/20 µL Inquire
01-03-00500 TERMIPol® DNA Polymerase, 5 U/µL, thermostable DNA polymerase that has increased efficiency for incorporating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides, supplied with a reaction buffer and 100 mM MgCl2, 500 U Inquire
01-03-02000 TERMIPol® DNA Polymerase, 5 U/µL, thermostable DNA polymerase that has increased efficiency for incorporating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides, supplied with a reaction buffer and 100 mM MgCl2, 2000 U Inquire
01-06-00500 HOT TERMIPol® DNA Polymerase, 5 U/µL, 500 U Inquire
01-06-02000 HOT TERMIPol® DNA Polymerase, 5 U/µL, 2000 U Inquire
02-21-00100 Solis BioDyne dNTP Set, chemically synthesized, 99% purity determined by HPLC, separate vials of dATP, dTTP, dGTP and dCIP at 100 mM concentration, 4 × 25 µmol Inquire
02-21-00400 Solis BioDyne dNTP Set, chemically synthesized, 99% purity determined by HPLC, separate vials of dATP, dTTP, dGTP and dCIP at 100 mM concentration, 4 × 100 µmol Inquire
02-31-00020 Solis BioDyne dNTP Mix, chemically synthesized, 99% purity determined by HPLC, one solution of dATP, dTTP, dGTP and dCIP at 20 mM concentration each, 20 µmol Inquire
02-31-00100 Solis BioDyne dNTP Mix, chemically synthesized, 99% purity determined by HPLC, one solution of dATP, dTTP, dGTP and dCIP at 20 mM concentration each, 100 µmol Inquire
02-41-00025 Solis BioDyne dUTP vial, at 100 mM concentration, 25 µmol Inquire
07-11-00050 DNA Ladder 100 bp ready to load molecular weight markers for DNA fragment size determination on gel electrophoresis, supplied in a loading buffer, contains 13 discrete DNA fragments ranging from 100 bp to 3,000 bp, stable at ambient temperature, 50 µg Inquire
07-12-00050 DNA Ladder 1 kb ready to load molecular weight markers for DNA fragment size determination on gel electrophoresis, supplied in a loading buffer, I kb contains 13 discrete DNA fragments ranging from 300 bp to 10,000 bp, stable at ambient temperature, 50 µg Inquire
07-01-00001 6x DNA Loading Dye Buffer Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 1 mL Inquire
07-01-00010 6x DNA Loading Dye Buffer Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 10 mL Inquire
07-02-00001 6x DNA Loading Dye Buffer Double Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 1 mL Inquire
07-02-00010 6x DNA Loading Dye Buffer Double Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 10 mL Inquire
07-03-00001 6x DNA Loading Dye Buffer Orange and Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 1 mL Inquire
07-03-00010 6x DNA Loading Dye Buffer Orange and Blue, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 10 mL Inquire
07-04-00001 6x DNA Loading Dye Buffer Orange, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 1 mL Inquire
07-04-00010 6x DNA Loading Dye Buffer Orange, to prepare DNA markers and samples for loading on agarose or polyacrylamide gels, Orange G for the analysis of small DNA molecules that have no DNA masking during gel exposure to UV light, Bromophenol Blue and Xylene Cyanol FF dark blue color make pipetting visually easy, 10 mL Inquire
05-16-00010 10x GC-rich Enhancer, PCR additive for difficult GC-rich templates, optimized solution modifies melting behavior of nucleic acids, enhances amplification of suboptimal PCR systems with high degree of secondary structures and GC-rich regions, 10x GC-rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non-specific amplification occur, 1 mL Inquire
05-16-00050 10x GC-rich Enhancer, PCR additive for difficult GC-rich templates, optimized solution modifies melting behavior of nucleic acids, enhances amplification of suboptimal PCR systems with high degree of secondary structures and GC-rich regions, 10x GC-rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non-specific amplification occur, 5 mL Inquire
05-16-00200 10x GC-rich Enhancer, PCR additive for difficult GC-rich templates, optimized solution modifies melting behavior of nucleic acids, enhances amplification of suboptimal PCR systems with high degree of secondary structures and GC-rich regions, 10x GC-rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non-specific amplification occur, 20 mL Inquire
05-11-00025 Magnesium Chloride (MgCl2), 25 mM, concentration should be optimized according to reaction conditions (primer, template, dNTP, polymerase concentration. 2.5 mL Inquire
05-11-00050 Magnesium Chloride (MgCl2), 25 mM, concentration should be optimized according to reaction conditions (primer, template, dNTP, polymerase concentration. 5 mL Inquire
05-11-00200 Magnesium Chloride (MgCl2), 25 mM, concentration should be optimized according to reaction conditions (primer, template, dNTP, polymerase concentration. 20 mL Inquire
Water-025 PCR Grade Water, deionized autoclaved, suitable for use in experiments that require nuclease-free water, prepared without chemical additives, DNase, RNase and nuclease-free, 25 mL Inquire
Water-100 PCR Grade Water, deionized autoclaved, suitable for use in experiments that require nuclease-free water, prepared without chemical additives, DNase, RNase and nuclease-free, 100 mL Inquire
Water-500 PCR Grade Water, deionized autoclaved, suitable for use in experiments that require nuclease-free water, prepared without chemical additives, DNase, RNase and nuclease-free, 500 mL Inquire