Biomass Sensors, Hamilton Incyte Capacitance Viable Cell Density and Dencytee Optical Density Total Cell Density
Overview
The Hamilton Incyte viable cell density measurement is based on capacitance. Viable cells behave like little capacitors and their polarization and depolarization in an alternating electrical field is measured. The signal can be correlated to the bio-volume. Only viable cells can be polarized in the alternating electrical field so this method is insensitive to cell debris as well as to microcarriers.
A measuring loop consists of an Incyte sensor that is available in different dimensions, an Incyte pre-amlifier and an Arc View controller. The Arc View can display both parameters at once using the 2 (Arc View 265) or 4 (Arc View 465, 465 XL) channel inputs. Additionally Arc pH and DO sensors with a wireless module can be displayed on the controller giving the ability to see all relevant parameters of a cell culture or fermentation on one screen. The Incyte sensor and pre-amplifier are sold as a calibrated Incyte Unit but are also available as spare parts.
The Hamilton Dencytee total cell density measurement is based on the optical density of a suspension at NIR wavelengths. All particles and molecules that scatter the NIR light will be detected and can be correlated to the total cell density. The Dencytee sensor keeps the light intensity at the detector constant leading to a wide linearity range.
A measuring loop consists of a Dencytee sensor with an optical path length of 5 mm, a Dencytee pre-amplifier and the Arc View controller. The controller can display both parameters at once using the 2 (Arc View 265) or 4 (Arc View 465, 465 XL) channel inputs. Additionally Arc pH and DO sensors with a wireless module can be displayed on the controller giving the ability to see all relevant parameters of a cell culture or fermentation on one screen. The Denytee sensor and pre-amplifier are sold as a Dencytee Unit but are also available as spare parts.
Features of the Incyte and Dencytee Cell Density Sensors:
The capacitance based Incyte cell density sensors give detail insight into cell culture and fermentation
Capacitance sensors are insensitive to micro-carriers, gas bubbles, cell debris and other particles in suspension
The Dencytee optical density sensors are based on turbidity, or optical density, measured at Near Infra-Red Wavelengths (NIR)
Sold as a Unit, consisting of an Incyte or Dencytee sensor and an individual pre-amplifier
An Arc View Controller is required, to display two parameters at once, using the 2 channel (Arc View 265) or 4 channel (Arc View 465, 465XL) inputs
Additional Arc pH and DO sensors with a wireless module can be used with an Arc View controller to see all relevant parameters of a cell culture or fermentation on one screen
Specifications (Click here):
Biomass Sensors, Hamilton Incyte Capacitance Viable Cell Density and Dencytee Optical Density Total Cell Density
Specifications
Model:
Incyte Unit DN25 – DG BE
Incyte Unit DN25 – DG
Incyte Unit DN25 – SG
Incyte Unit DN12
Dencytee Unit DN12
Length: (mm)
54
52
72
120, 220, 320, 420
120, 220, 320, 420
Accuracy 1: (mammalian cells/mL)
± 5×104
± 5×104
± 5×104
± 5×104
-
Accuracy 2: (g/L)
± 0 05 dry weight (fermentation)
± 0 05 dry weight (fermentation)
± 0 05 dry weight (fermentation)
± 0 05 dry weight (fermentation)
-
Autoclavable:
yes
yes
yes
yes
yes
CIP:
yes
yes
yes
yes
no
Conductivity range: (mS/cm)
2 to 50 (medium)
2 to 50 (medium)
2 to 50 (medium)
2 to 50 (medium)
-
Density range: (AU)
-
-
-
-
0 - 5
Diameter: (mm)
-
-
-
12
12
Light source:
-
-
-
-
LED
Measurement principle:
-
-
-
-
NIR
Measuring range 1: (mammalian cells/mL)
5×105 to 8×109
5×105 to 8×109
5×105 to 8×109
5×105 to 8×109
5×105 to 7×108
Measuring range 2: (g/L)
5 to 200 dry weight (fermentation)
5 to 200 dry weight (fermentation)
5 to 200 dry weight (fermentation)
5 to 200 dry weight (fermentation)
0.5 to 300 dry weight (fermentation)
O-ring material:
EPDM (FDA approved, USP Class VI)
EPDM (FDA approved, USP Class VI)
EPDM (FDA approved, USP Class VI)
EPDM (FDA approved, USP Class VI)
EPDM (FDA approved, USP Class VI)
OP (SG): mm
27
27
27
-
-
OP (DG): mm
27 and 47
27 and 47
27 and 47
-
-
Operating temperature range: (°C)
0 to 60
0 to 60
0 to 60
0 to 60
0 to 60
Optical path length: (mm)
-
-
-
-
5
Optical window:
-
-
-
-
sapphire
Pressure range: (bar)
0 to 3
0 to 3
0 to 3
0 to 3
0 to 10
Process connection:
G 1-1/4" (Ingold or Sartorius)
G 1-1/4" (Ingold or Sartorius)
G 1-1/4" (Ingold or Sartorius)
PG13.5
PG13.5
Steam sterilizable:
Yes, maximum temperature 140°C
Yes, maximum temperature 140°C
Yes, maximum temperature 140°C
Yes, maximum temperature 140°C
Yes, maximum temperature 135°C
Wavelength: (nm)
-
-
-
-
8
Wetted parts:
Stainless steel 1.4435, PEEK (FDA approved, USP class VI), Platinum,
Stainless steel 1.4435, PEEK (FDA approved, USP class VI), Platinum,
Stainless steel 1.4435, PEEK (FDA approved, USP class VI), Platinum,
Stainless steel 1.4435, PEEK (FDA approved, USP class VI), Platinum,
Incyte Viable Cell Density Unit DN25 – DG BE Capacitance Sensor, 54 mm length
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243711
Incyte Viable Cell Density Unit DN25 – DG Capacitance Sensor, 52 mm length
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243710
Incyte Viable Cell Density Unit DN25 – SG Capacitance Sensor, 72 mm length
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243700
Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 120 mm length, 12 mm Ø
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243701
Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 220 mm length, 12 mm Ø
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243702
Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 320 mm length, 12 mm Ø
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243703
Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 420 mm length, 12 mm Ø
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243755
Dencytee Total Cell Density Unit DN12-120 Optical Density Sensor, 120 mm length, 12 mm Ø
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243756
Dencytee Total Cell Density Unit DN12-220 Optical Density Sensor, 220 mm length, 12 mm Ø
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243757
Dencytee Total Cell Density Unit DN12-320 Optical Density Sensor, 420 mm length, 12 mm Ø
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243758
Dencytee Total Cell Density Unit DN12-420 Optical Density Sensor, 420 mm length, 12 mm Ø
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243800
Arc View 265 Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 2 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 250×220×110 mm
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243801
Arc View 465 Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 4 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 250×220×110 mm
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243802
Arc View 465XL Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 4 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 443×114×364 mm
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243810
PC AUX to Analog Output Box, 4 measuring channels, display information on a computer, USB input and output
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243873
Sensor cable, 40 m, M12.5 Pole male/female process connection
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243872
Sensor cable, 20 m, M12.5 Pole male/female process connection
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243871
Sensor cable, 10 m, M12.5 Pole male/female process connection
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243870
Sensor cable, 5 m, M12.5 Pole male/female process connection
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243842
Solution B for Incyte Calibrations, 180 mL sodium sulphate solution for 2 calibrations from 2 - 50 mS
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243740
Validation and Calibration Kit for Incyte Sensors, includes Solution B (243842), Standard conductivity solution 12,85 mS and Calibrator to check the internal electronics of the Incyte Pre-Aplifier
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238988
Conductivity Standard, 12880 μS/cm, Basic Line, Accuracy ± 1%, Stability 18 months, Value at 25°C 12880 µS/cm, 500 mL Calpack bottle
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243850
Analog Output Box, transfer measurements from Arc View Controller to the Process Control System, 4 outputs for Cell Density, 4 outputs for Conductivity
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243852
Cable for Analog Outputs Box, 10 m. M12 5 Pole / DIN-RAIL process connection
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243851
Cable for Analog Output Box, 5 m. M12 5 Pole / DIN-RAIL process connection
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243822
Incyte License required to use Incyte sensors with ARC Controllers
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243823
Incyte Scan License for optional measurement at 17 additional frequencies to analyze a bioprocess in more detail
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243824
Dencytec License required to use Dencyte sensors with ARC Controllers
