Cell Counters, Automated Brightfield, Fluorescence, Yeast and Microbial
Overview
Cell counting is a fundamental quality control procedure in research. It’s simple. It’s straightforward. But it’s also error-prone, time-consuming, and highly subjective.
The Logos Biosystems automated cell counters are equipped with high quality optics and sophisticated software to supply cell concentration and viability data with incredible speed, accuracy, and reliability.
It’s as simple as pressing COUNT.
The LUNA™, LUNA-II™, LUNA-FL™, LUNA-STEM™, LUNA-II YF™ and QUANTOM Tx™ automated cell counters are compatible with multiple cell types, from PBMCs to bacteria and yeast. They feature customizable protocols to adjust cell detection parameters to count and detect specific cell types. Advanced declustering algorithms detect individual cells in clusters.
Prepare samples and the counters do the rest, including tagging counted cells so that results can be immediately checked and providing for simple record keeping.
image-based automated cell counters act as eyes and a microscope, using image-analysis software and a computer to produce consistently accurate and reliable cell counts.
The six Logos Biosystems LUNA™, LUNA-II™, LUNA-FL™, LUNA-STEM™, LUNA-II YF™ and QUANTOM Tx™ cell counters produce accurate and reproducible cell counts with a great linearity, low technical variability, lower costs and great convenience. Review the features to determine which best suits an application.
Features of the Logos automated cell counters:
Six models to match with budget and laboratory requirements
Compatible with multiple cell types
Customizable protocols
Advanced cluster analysis
Monitor counted cells
Simple record keeping, printed or to PC
Easy to use, just prepare samples
Follow the steps: Stain, Load, Count, Review and Export
No more subjectivity, variability or wasted time
Use convenient disposable slides or economical reusable slides
Features of the Logos LUNA™ Automated Cell Counters:
Classic first model created Logos reputation
Automated Brightfield cell counting
Small footprint
Results in as little as 7 seconds
Consistently distinguish between live and dead cells from debris
Live cells are tagged with green circles and dead cells are tagged with red circles
Advanced cluster analysis
IQ/OQ Protocol available
Features of the Logos LUNA-II™ Automated Cell Counters:
Autofocusing liquid lens
Results in as little as 15 seconds
Consistently distinguish between live and dead cells from debris
Live cells are tagged with green circles and dead cells are tagged with red circles
Advanced cluster analysis
IQ/OQ Protocol available
Features of the Logos LUNA-FL™ Automated Dual Fluorescence Cell Counter:
Powerhouse with four counting modes
Brightfield and dual fluorescence optics
Sensitive detection of most cell types, excluding bacteria
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
Fluorescence results: 3 images images captured and analyzed within 30 seconds
Brightfield results: 1 image captured and analyzed within 10 seconds
IQ/OQ Protocol available
Features of the Logos LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells and SVF:
Takes adipose-derived stem cell and SVF samples to count live and dead nucleated cells as well as non-nucleated cells
Equipped with dual fluorescence and brightfields optics
Can distinguish between cells and debris
Produces results in as little as 30 seconds
Reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability and average cell size
High resolution image with live cells labeled with green circles and dead cells with red circule can be used to confirm data visually
IQ/OQ Protocol available
Features of the Logos LUNA-II YF™ automated Yeast Cell Counters:
Cell counting and declustering algorithm gives results in 15 aeconds
Dual fluorescence optics with autofocusing liquid lens
Can count with the precision and consistency of a flow cytometer
Unlike a flow cytometer, can count severely clumped cells
Independently tested on more than 30 brewing yeast products
Features of the Logos QUANTOM Tx™ Automated Microbial Cell Counters:
No culturing required
In less than 30 seconds scan up to 10 fields of view to produce accurate single bacterial cell counts
Advanced software can handle varying shapes, sizes and arrangement of bacteria
Can count bacterial cells in even the tightest clusters
No estimating based on colony forming units or turbidity
Total and viable cell counting
QUANTOM™ Total Cell Staining Dye permeates both gram positive and gram negative bacteria, dead or alive
Stained cells are mixed with loading buffer into QUANTOM™ M50 Cell Counting Slides, then spun the QUANTOM Centrifuge to immobilize and evenly distribute cells in the counting chamber
Specifications (Click here):
Cell Counters, Automated Brightfield, Fluorescence, Yeast and Microbial
Yeast Viability Kit I, with Fluorescein Diacetate Stain, Propidium Iodide Stain for Yeast. Fluorescence Signal Enhancer 1, Cell Dilution Buffer
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Q13501
QUANTOM™ Total Cell Staining Kit, with Total Cell Staining Dye, Total Cell Staining Enhancer,, Cell Loading Buffer I
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Q13502
QUANTOM™ Viable Cell Staining Kit, with Viable Cell Staining Dye, Viable Cell Dilution Buffer and Cell Loading Buffer I and Dimethyl Sulfoxide
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L12001
Disposable LUNA Cell Counting Slides for brightfield cell counting, polystyrene, W×D×H = 25×75×2.4 mm, chamber depth 100 µm, loading volume 10 µL pkg of 50
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L12005
Disposable PhotonSlides™, ultra-low autofluorescence for fluorescence cell counting, Poly(methyl methacrylate (PMMA), W×D×H = 25×75×2.4 mm, chamber depth 100 µm, loading volume 10 µL, pkg of 50
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Q12001
Disposable QUANTOM M50 Cell Counting Slides, ultra-low autofluorescence for fluorescence cell counting, Poly(methyl methacrylate (PMMA), W×D×H = 25×75×2.4 mm, chamber depth 50 µm, loading volume 5 µL, pkg of 50
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L12011
LUNA Reusable Slides, for brightfield and fluorescence cell counting, aluminum alloy and glass, W×D×H = 25×75×2.4 mm, chamber volume 10 µL comes with one resuable glass coverslip W×D×H = 20×15×0.6 mm,
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P10001
LUNA™ Printer
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B13101
LUNA™ Standard Beads, for calibrating brightfield cell counting, 2×1 mL
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F23102
LUNA™ Fluorescence Calibration Beads for calibrating fluorescence cell counting.
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Q13102
UANTOM™ Calibration Beads for calibrating the QUANTOM Tx™ Microbial Cell Counter
