Columns, SMT Phenyl and Phenyl-Hexyl HPLC Overview
SMT Phenyl and Phenyl-Hexyl columns provide unique selectivity for aromatic compounds when compared to other reversed-phase packings such as C18 and C8. The π-electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to less or non-aromatic analytes.
Features of SMT Phenyl and PHENYL-Hexyl Columns:
Available in three types, Phen1 (one phenyl per ligand), Phen2 (two phenyls per ligand) and PhenH (phenyl-hexyl chain configuration)
Preferential retention of aromatic compounds
Complimentary to other reversed-phase materials such as C18, C8, and C4
Ideal for the separation of proteins, peptides and other biomolecules.
Particle sizes of 5 and 10 µm are stock, others available
Pore sizes of 100 and 300Å are stock, others available
A comparison of a typical selectivity obtainable in the separation of a mixture containing aliphatic and aromatic compounds using of SMT-Phen1, SMT-Phen2, SMT-PhenH and SMT-C18 columns
Aromatic compounds: Steroids
Column:
SMT Phen1-5-100-15
Solutes:
1 = prednisolone
Eluent:
Methanol: Water (60:40)
Flow:
1.0 mL/min
Detector:
UV; 254nm
Temp:
30° C
Remarks:
Aromatic compounds: Steroids
Column:
SMT OD-5-100-15
Solutes:
1 = prednisolone
Eluent:
Methanol: Water (60:40)
Flow:
1.0 mL/min
Detector:
UV; 254nm
Temp:
30° C
Remarks:
Aromatic compounds: Steroids
Column:
SMT OD-5-100-15
Solutes:
1 = prednisolone
Eluent:
Methanol: Water (60:40)
Flow:
1.0 mL/min
Detector:
UV; 254nm
Temp:
30° C
Remarks:
Aromatic compounds: Steriods
Column:
SMT Phen1-5-100-15
Solutes:
1 = prednisolone
Eluent:
Methanol: Water (60:40)
Flow:
1.0 mL/min
Detector:
UV; 254nm
Temp:
30° C
Remarks:
Aromatic compounds: Steriods
Column:
SMT Phen1-5-100-15
Solutes:
1 = prednisolone
Eluent:
Methanol: Water (60:40)
Flow:
1.0 mL/min
Detector:
UV; 254nm
Temp:
30° C
Remarks:
Specifications (Click here):
Columns, SMT Phenyl and Phenyl-Hexyl HPLC
Model:
Typical SAM Phen1 column Typical SAM Phen1 column Typical SAM Phen2 column Typical SAM Phen2 column Typical SAM PhenH column Typical SAM PhenH column
5 µm Silica: (Å)
100
300
100
300
100
300
Surface area: (m2 /g)
340
90
340
90
340
90
Carbon: (%)
7.1
3.1
8.4
3.6
5.2
3.4
Coverage: (moles/m2 )
7.3
7.2
7.2
7.2
7.2
7.2
Stock particle sizes: (µm)
5, 10
5, 10
5, 10
5, 10
5, 10
5, 10
Stock pore sizes: (Å)
100, 300
100, 300
100, 300
100, 300
100, 300
100, 300
Number Description Price $
Phen1-5-100-5
SMT Phenyl-1 Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
Phen1-5-100-10
SMT Phenyl-1 Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D
646.55
Phen1-5-100-15
SMT Phenyl-1 Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
Phen1-5-100-25
SMT Phenyl-1 Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
Phen1-5-300-5
SMT Phenyl-1 Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D
579.15
Phen1-5-300-10
SMT Phenyl-1 Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D
646.55
Phen1-5-300-15
SMT Phenyl-1 Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
Phen1-5-300-25
SMT Phenyl-1 Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
Phen1-10-100-5
SMT Phenyl-1 Column, 10 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
Phen1-10-100-10
SMT Phenyl-1 Column, 10 µm, 100Å, 100 mm L x 4.6 mm I.D
646.55
Phen1-10-100-15
SMT Phenyl-1 Column, 10 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
Phen1-10-100-25
SMT Phenyl-1 Column, 10 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
Phen1-10-300-15
SMT Phenyl-1 Column, 10 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
Phen1-10-300-25
SMT Phenyl-1 Column, 10 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
Phen2-5-100-5
SMT Phenyl-2 Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
Phen2-5-100-10
SMT Phenyl-2 Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D
646.55
Phen2-5-100-15
SMT Phenyl-2 Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
Phen2-5-100-25
SMT Phenyl-2 Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
Phen2-5-300-5
SMT Phenyl-2 Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D
579.15
Phen2-5-300-10
SMT Phenyl-2 Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D
646.55
Phen2-5-300-15
SMT Phenyl-2 Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
Phen2-5-300-25
SMT Phenyl-2 Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
Phen2-10-100-5
SMT Phenyl-2 Column, 10 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
Phen2-10-100-10
SMT Phenyl-2 Column, 10 µm, 100Å, 100 mm L x 4.6 mm I.D
646.55
Phen2-10-100-15
SMT Phenyl-2 Column, 10 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
Phen2-10-100-25
SMT Phenyl-2 Column, 10 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
Phen2-10-300-15
SMT Phenyl-2 Column, 10 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
Phen2-10-300-25
SMT Phenyl-2 Column, 10 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
PhenH-3-100-5
SMT Phenyl-H Column, 3 µm, 100Å, 50 mm L x 4.6 mm I.D
622.05
PhenH-3-100-10
SMT Phenyl-H Column, 3 µm, 100Å, 100 mm L x 4.6 mm I.D
694.55
PennH-3-100-15
SMT Phenyl-H Column, 3 µm, 100Å, 150 mm L x 4.6 mm I.D
738.05
PhenH-5-100-5
SMT Phenyl-H Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
PhenH-5-100-10
SMT Phenyl-H Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D
646.65
PennH-5-100-15
SMT Phenyl-H Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
PennH-5-100-25
SMT Phenyl-H Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
PhenH-5-300-5
SMT Phenyl-H Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D
579.15
PhenH-5-300-10
SMT Phenyl-H Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D
646.55
PennH-5-300-15
SMT Phenyl-H Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
PennH-5-300-25
SMT Phenyl-H Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
PhenH-10-100-5
SMT Phenyl-H Column, 10 µm, 100Å, 50 mm L x 4.6 mm I.D
579.15
PhenH-10-100-10
SMT Phenyl-H Column, 10 µm, 100Å, 100 mm L x 4.6 mm I.D
646.65
PennH-10-100-15
SMT Phenyl-H Column, 10 µm, 100Å, 150 mm L x 4.6 mm I.D
754.65
PennH-10-100-25
SMT Phenyl-H Column, 10 µm, 100Å, 250 mm L x 4.6 mm I.D
835.65
PhenH-10-300-5
SMT Phenyl-H Column, 10 µm, 300Å, 50 mm L x 4.6 mm I.D
579.15
PhenH-10-300-10
SMT Phenyl-H Column, 10 µm, 300Å, 100 mm L x 4.6 mm I.D
646.65
PennH-10-300-15
SMT Phenyl-H Column, 10 µm, 300Å, 150 mm L x 4.6 mm I.D
754.65
PennH-10-300-25
SMT Phenyl-H Column, 10 µm, 300Å, 250 mm L x 4.6 mm I.D
835.65
