SMT’s unique bonding method produces very high ligand density silica based octyl (C8) column packing material that results in chromatographic properties unlike any other silica-based C8 column support.
The SMT C8 columns are designed for optimum peak shape, peak capacity and high chromatographic efficiency for both acidic and basic solutes.
SMT C8 columns are very stable at extreme pH conditions and high temperatures. The column is strongly recommended for the separation of most basic solutes that have high concentrations of very hydrophobic functional groups. A C8 column is usually the second column of choice after C18 for method developments using reversed-phase chromatographic separation. When compared to other columns such as C4, CN, phenyl, or an amino bonded phase, the C8 is the most hydrophobic.
Although C18 remains the most widely used, the C8 phase represents a good compromise phase. C8 phase normally provides equivalent selectivity; it is not too hydrophobic, and yet it retains many compounds on the basis of interaction with their hydrophobic groups. C8 phases are good choices when too much organic solvent is required to elute the analytes of interest (especially highly hydrophobic molecules) from a C18 phase. The use of C8 columns reduces retention time and consumption of organic solvents
SMT O Series is the SAM-C8 phase with the highest functional ligand coverage confirmed with carbon analysis results of 12% carbon load. In this series, a very high density of the functional ligand, octyl molecule or C8, is achieved through meticulous mixture of the C8 with proprietary spacer molecules to insure maximum coverage. These phases are very hydrophobic and are designed to tolerate usage in mildly aggressive pH conditions and high temperatures. SMT SAM-C8 columns are designed to withstand a pH range of 1-10. The columns are generally 50% more stable than those of competitors used under similar conditions.
SMT OL Series SAM C8 HPLC Columns
The SMT OL Series SAM-C8 phase with the lowest functional ligand coverage confirmed with carbon analysis results of 6% carbon load. In these series, the proportional ratio of the functional ligand, octyl or C8 molecule, mixed with the proprietary spacer molecule, is reduced. The result is a packing material with maximum coverage but much lower functional ligand density. Low density C8 packing material ensures faster mass transfer of solutes during separation. These phases have low hydrophobicity. The spacer molecules protect the substrate from aggressive pH conditions and impact unique selectivity compared with other C8 phases.
Features of SMT OL Series SAM C8:
Fast mass transfer and high efficiency for the separation of highly hydrophobic molecules
Unique selectivity compared with other C8 phases
Higher population of spacer molecules provides unique mixed-mode effect for the selectivity
Stable bonding for long column lifetimes
Particle sizes of 5 and 10 µm are stock, others available
Pore sizes of 100 and 300Å are stock, others available
