Cryostats, the Bright OTF5000 Floor Standing Basic, LS and HS Models
Overview
The Bright OTF5000 floor standing biological cryostat is a medical device used to slice tissue and other samples very finely for analysis. The instrument is essentially a stationary freezer (the cryostat) capable of reaching temperatures within the range of −20 to −45 °C, with a rotary microtome incorporated into it that cuts histological and other samples extremely finely.
The microtome operates by use of an external wheel or lever which turns the blade and slices the sample. The Bright OTF5000 is suitable for an endless range of applications and is capable of cutting a wide diversity of specimens from undecalcified bone to brain, resins, plastics and plant tissue as well as more usual soft tissues.
The Bright OTF5000 cryostat range is suitable for both research and clinical operation and can be used with either solid knives or disposable blades in the microtome. The OTF5000 range is available in three standard models, with a long list of options and accessories to accommodate every sample slicing need in a laboratory.
All non-urgent functions are on a side panel, away from the essential systems..
Allowing full anti-roll plate adjustment for perfect results coupled with long lasting temperature stability, the OTF5000 is packed with features. Inquire about low cabinet height option, glove port, thin section conversions, large area sectioning and uv decontamination kits. A variety of holders, knives, honing supplies and lubricants are available.
All OTF5000 Cryostats come with Easi-set anti-roll plates (37mm, 50mm and 75mm), circular object holders (2 × 22mm, 1 × 37mm), 200mL bottle of low temperature oil, set of Allen keys, 113mL bottle of Cro-M-Bed, debris brush and debris tray.
The Bright 2212 Starlet compact bench top portable cryostat with radial, flat cutting rotary microtome is also available from Rose Scientific for use in laboratories where space is at a premium.
Features of the Bright OTF5000 Cryostats:
Three models, Basic, LE and LS
Uses solid knives or disposable blades with lever release disposable blade holder
Automatic evaporator defrost supplied as standard
The OTF5000 Basic includes Quick Freezer, Internal shelves, Evaporator defrost, Manual cutting system, 5040 microtome with fixed head, Heated window surround, Window demist system, Analog defrost clock, Side mounted non urgent controls and a full set of accessories
The OTF5000 LS specification adds 2 speed motorized advance and rewind, Fine object orientation and Cryomatic specimen temperature control
The OTF5000 HS specification adds to the LS features a 6 digit section counter, Dual refrigeration and Motor drive
All models are supplied with three anti-roll plates, two circular object holders, bottle of low temperature oil, Allen keys, bottle of Cro-M-Bed, debris brush and debris tray
Specifications (Click here):
Cryostats, the Bright OTF5000 Floor Standing Basic, LS and HS Models
9 position, running at 10 below chamber, ultra low –80 version can be factory fitted at extra cost
9 position, running at 10 below chamber, ultra low –80 version can be factory fitted at extra cost
9 position, running at 10 below chamber, ultra low version cannot be fitted
Shelves:
3 internal, 1 external
3 internal, 1 external
3 internal, 1 external
Evaporator defrost:
Automatic with analog clock
Automatic with analog clock
Automatic with analog clock
Cutting stroke: (mm)
Manual with balanced handwheel
Manual with balanced handwheel
Manual with balanced handwheel
Cutting system:
5040 rotary microtome with beryllium hinges
5040 rotary microtome with beryllium hinges
5040 rotary microtome with beryllium hinges
Retraction on return stroke: (µm)
50
50
50
Object orientation: (°)
-
Fine, ± 8 in horizontal and vertical axes, 360 rotation
Fine, ± 8 in horizontal and vertical axes, 360 rotation
Motorized advance and rewind, 2 speed, autoreset: (µm/s)
-
116 and 164
116 and 164
Specimen temperature control:( °C)
-
-5 to minimum chamber temperature
-5 to minimum chamber temperature
Knife angle adjustment:(°)
25
25
25
Section thickness range, standard: (µm)
0.5 to 30 in 0.5 increments
0.5 to 30 in 0.5 increments
0.5 to 30 in 0.5 increments
Maximum head advance: (mm)
5.6
5.6
5.6
Maximum knife block adjustment, coarse control: (mm)
44
44
44
Normal working height: (mm)
990
990
990
Low working height: (mm)
890
890
890
Weight: (kg)
120
120
140
Dimensions with handle, low height model: (mm)
755×935×1200
755×935×1200
840×935×1200
Dimensions with handle, standard height model: (mm)
755×935×1300
755×935×1300
840×935×1300
Number
Description
Price $
QTF5000-002BASIC
OTF5000 Basic Cryostat fitted with standard knife holder assembly, Anti-roll system, 50230 solid microtome knife (22°angle), 110/115V, 50/60Hz
33,005.70
QTF5000-004BASIC
OTF5000 Basic Cryostat fitted with Quick Release Disposable Blade Holder, Anti-roll plate, Ten disposable blades, 110/115V, 50/60Hz
34,002.35
QTF5000-002LS
OTF5000-LS Cryostat fitted with standard knife holder assembly, Anti-roll system, 5023 solid microtome knife (22°angle), Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, 110/115V, 50/60Hz
35,760.40
OTF5000-004/LS
OTF5000-LS Cryostat fitted with Quick Release Disposable Blade Holder, complete with anti-roll plate and ten feather blades, Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, 110/115V, 50/60Hz
37,054.15
OTF5000-002HS
OTF5000-HS Cryostat fitted with standard knife holder assembly, Anti-roll system, 50230 solid microtome knife (22°angle), Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, Section counter (6-digit LCD display), Dual refrigeration -40°C chamber temperature, Motor drive sectioning and footswitch, 110/115V, 50/60Hz
44,863.30
OTF5000-004HS
OTF5000-HS Cryostat fitted with Quick Release Disposable Blade Holder, Anti-roll plate and ten feather blades, Motorized Advance Rewind (2-speed, 116µm/s and 164µm/s), 50539 Object Orientation, Cryomatic Specimen Temperature Control fast reacting from -5°C to minimum chamber temperature, Section counter (6-digit LCD display), Dual refrigeration -40°C chamber temperature, Motor drive sectioning and footswitch, 110/115V, 50/60Hz
45,833.75
53581
Bright Cryo-m-Bed, 113 mL bottle, blend of polyviol alcohol 217, thymol and water, for imbedding many different types of tissue over 18ºC to –20ºC and wider temperature range, pkg/6
430.10
57713
Bright Cryospray 134, 340g (300 mL) cans, aerosol freezing agent, 80 – 100% Tetrafluoroethane, 155 mm nozzle delivers jet at - 52°C, pkg/12 (not available because of shipping issues, inquire about alternatives)
Inquire
50539
Orientating object holder, with fine adjustment screws, factory fitted
1,501.90
50657
Orientating object holder, without fine adjustment screws, factory fitted
3,474.15
51637
Quick release hardboard holder, without orientation, for 5040-02
3,356.86
51637-1
Quick release hardboard holder, with orientation, for 5040-01
Inquire
50533
Quick release wax cassette holder, without orientation, for 5040-02
3,356.85
50209
Triangular glass knife holder, fixed
944.15
50681
Triangular glass knife holder, orientating. These accept 25mm high Latta-Hartmann knives with edges up to 15mm
2,065.40
52985
CO2 (gas) object holder and knife freezer
3,474.15
50208
Sloped (20º) knife holder
5,761.50
50213
Ralph knife holder
1,644.50
50214
Ralph knife holder with section float-out trough. For long edged glass knives with edges up to 38mm
1,751.45
52987
Illuminated magnifier
3,474.15
50230
Standard knife with box, C-profile, 189 x 27 x 10mm. 22º angle, tapped to accept holder for Shandon knife sharpener, safety cut-outs at each end, for 5030 and 5040 microtomes
882.05
50724
Knife sharpening back for 50230
313.95
50232-1
Hardened steel knife with box, C-profile, 160 x 35 x 9mm. 15º angle, suitable for large area sections
3,474.15
50210
Knife sharpening back for 50232-1
378.35
50207
Hardened steel knife with box, C-profile, 160 x 32 x 13mm. 23º, similar to 50232-1 but slightly heavier for tougher specimens
1,143.10
50211
Knife sharpening back for 50207
325.45
51675
Handle for 50230, 50232-1 and 50207
190.90
50234
Tungsten carbide tipped knife with box, 228 x 38 x 6mm. Suitable for cutting bone and hard plastics. needs to be resharpened at factory
2,970.45
52647
Stem for 50232-1 and 50234 knives.
146.05
52671
Knife sharpening kit. Includes hone, strop, oil, three grades of abrasive and instructions
1,367.35
57612
Coarse abrasive, 6g
75.90
57611
Fine abrasive, 6g
75.90
57610
Honing compound, 6g
75.90
50239
Clock oil, 6g
21.85
50300-1
Magnaplate non-adjustable anti-roll plate. Fits most knives and blades. picture shows sitting on a standard 50230 knife, package of 3 with a single removable handle
168.75
50140
Magnacut disposable blade system, with magnetic blade holder, for direct replacement of a solid knife system in a 5030 or 5040 microtome, includes 50 mm anti-roll plate, 10 blades, blade removal tool, micro-adjusting T-piece and stem, box and instructions
2,656.50
50133
Magnacut disposable blade system, with magnetic blade holder, for use without a fixed anti-roll plate, fits most cryostats, includes Magnaplate non-adjustable anti-roll plate, 10 blades, blade removal tool, box and instructions, picture is with Magnacut holder, disposable blade and Magnaplate non-adjustable anti-roll plate
2,544.95
50540
Magnacut disposable blade holder only
2,204.55
52727
Long-life disposable blades for Magnacut, C-profile, pkg/50
509.10
51564
Magnacut blade removing tool
37.95
50240
Feather blade holder with box
317.40
57903
Lever release Feather blade holder, accepts low profile S35 blades or similar, fits standard knife holders on many microtomes
Inquire
50241
Feather disposable blades, pack of 50
509.10
50260
5 - 300μm feed screw for non /Mar
3,069.35
50259
3 - 180μm feed screw for non /Mar
1,838.85
50258
2 - 120μm feed screw for non /Mar
1,675.55
50257
1 - 60μm feed screw for non /Mar
1,151.15
50255
0.5 - 30μm feed screw, supplied as standard for non /Mar
1,045.35
50256
0.2 - 12μm feed screw for non /Mar
1,386.90
50259-1
3 - 180μm feed screw for /Mar option
2,157.40
50258-1
2 - 120μm feed screw for /Mar option
1,997.55
50257-1
1 - 60μm feed screw for /Mar option
1,905.55
50255-1
0.5 - 30μm feed screw for Mar option, supplied as standard
1,367.35
50256-1
0.2 - 12μm feed screw for /Mar option
1,706.60
50727
Standard tubular holder, 22mm diameter, fits Bright 5030 microtome only
90.00
50734-1
Object holder, 22mm diameter, solid, suitable for most applications
133.40
50734-2
Object holder, 22mm diameter, solid, as 50734-1 but long type, gives greater clearance between specimen clamp and knife holder, ideal when multi-position glass slides are used
146.05
50735-1
Object holder, 37mm diameter, solid, suitable for larger specimens
292.10
50735-2
Object holder, 37mm diameter, solid, as 50735-1 but long type, suitable for larger specimens and gives extra clearance
305.90
50744
Object holder, 5mm diameter, solid, suitable for small specimens, particularly when orientation is required
133.40
50721
Object holder with stem, 50mm diameter, suitable for very large specimens
323.15
50741
Object holder with stem, 50 x 50mm square, suitable for very large specimens
374.90
50743
Object holder, 50 x 70mm
384.45
50221
Quick release object holder clamp, complete with 50 metal object discs
3,576.50
50203
Quick release object holder, fits into a standard vice clamp and takes 50690 metal discs
Cork object holder discs, 50 x 50mm square, pkg/50
215.05
50162
Adaptor, to accept Ames cryostat, saddle type object holders
870.55
52658
Vertical vice clamp
455.05
54791
Low temperature oil, 200ml bottle
123.05
54791-1
Low temperature oil, 4.54 litre drum
397.90
57355
Low temperature grease, 5g bottle
123.35
57344
Knife cleaning brush
258.75
57808
Anti static brush, 12mm
172.50
52531
Wax brush and debris tray
256.45
53573
Spare fuse set for 5040-001 or 5040-002 with 225MR
80.50
53574
Spare fuse set for 5040-001 or 5040-002 with 226MR
80.50
51695
Universal holder fits 5030 or 5040 microtomes that have fixed heads, can be fitted with object holders 50745, 50720, 50719, 50718 or 50712 to give coarse orientation
1,181.25
50745
Orienting object holder 5mm diameter, for small specimens
112.75
50720
Orienting object holder 22mm diameter, for medium size specimens
116.25
50719
Orienting object holder 37mm diameter, for larger specimens
176.25
50718
Orienting object holder 50mm diameter, for very large specimens
243.75
50712
Orienting object holder 50 × 50mm square, for very large specimens
255.00
50424
Easy-Set anti roll plate 37mm, side wings make it easy to adjust
112.50
50425
Easy-Set anti roll plate 50mm, side wings make it easy to adjust
116.25
50426
Easy-Set anti roll plate 75mm, side wings make it easy to adjust