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Columns, SMT MEB (Methyl, Ethyl and Butyl) C1, C2 and C4 Short Carbon Chain HPLC

  Overview

SMT manufactures MEB columns with short carbon chains for rapid analysis of basic, neutral, and mildly acidic drugs and small molecules and biomolecules. These columns provide excellent baseline resolution for small basic molecules including therapeutic drugs. The bonding scheme ensures proper deactivation substrate silanols and resistance to acid hydrolysis.

Reversed-phase HPLC has been a valuable tool for the separation of variety of molecules including organics, pharmaceuticals, drugs, biomolecules, and natural products. Most analyses are performed using long-chain C18 and C8. These bonded phases are very popular because of their ruggedness and stability in generally harsh mobile phase environments. However, extended separation time and low efficiency have also been observed in many molecules of interest. For example, in separation involving biomolecules such as hydrophobic proteins, excessive hydrophobic interaction may actually result in denaturing of the proteins.

MEB (Methyl, Ethyl, and Butyl) columns are particularly superior to other reversed-phases in the separation of compounds that show very strong hydrophobic interaction with hydrophobic stationary phases such as C18 and C8. MEB columns offer extremely fast mass transfer and high efficiency in the separation of these compounds. Specific applications include separation of proteins, peptides and food additives.

Features of SMT MEB Columns:
  • Three different MEB phases of varying carbon loads, for optimal selectivity
  • Rapid analysis of basic, neutral and mildly acidic drugs, small molecules and biomolecules
  • Excellent baseline resolution for small basic molecules, including therapeutic drugs
  • Particularly superior to other reversed-phases in the separation of compounds that show very strong hydrophobic interaction with hydrophobic stationary phases

SMT offers 3 different MEB phases of varying carbon loads for optimal selectivity.

MEB1 series: SMT MEB column with the shortest carbon length. The functional ligand is methyl, C1 with carbon analysis results of about 1% carbon load. In these series, a very high density of the functional ligand, methyl molecule or C1, is achieved through a novel method of molecular assembly that ensures maximum coverage of the short chain. These phases are the least hydrophobic of all the MEB columns. Offers selectivity for polar and nonpolar pharmaceuticals, natural products, very hydrophobic proteins and biomolecules. Longer column lifetime than traditional C1 columns.

MEB2 series: SMT MEB column with only two carbon chains. The functional ligand is ethyl, C2 with carbon analysis results of 2% carbon load. In these series, a very high density of the functional ligand, ethyl molecule or C2, is achieved through a novel method of molecular assembly that ensures maximum coverage. These phases offer medium hydrophobicity when compared with all the other MEB phases. Offers selectivity for polar and nonpolar pharmaceuticals, natural products, very hydrophobic proteins and biomolecules Longer column lifetime than traditional C1 columns.

MEB4 series: SMT MEB column with four carbon chains. The functional ligand is butyl, C4 with carbon analysis results of about 4% carbon load. In these series, a very high density of the functional ligand, butyl molecule or C4, is achieved through a novel method of molecular assembly that ensures maximum coverage. These phases offer the highest hydrophobicity when compared with all the other MEB phases. Designed to tolerate mildly aggressive pH conditions that may hamper usage life of MEB1 and MEB2 columns. Excellent peak symmetry; offers very good selectivity for polar and moderately nonpolar pharmaceuticals and biomolecules. Longer column lifetime than traditional C4 columns. Virtually no nonspecific adsorption of proteins

MEB1 and Therapeutic Drugs: Biaxin®
Column: MEB1-5-100-5
Solutes: 1 = Unown
2 = Clarithromycin
Eluent: CAN: 0.01M ammonium formate buffer 90:10 (v:v)
Flow: 1.0 mL/min
Detector: UV; 280nm
Temp: 30° C
Remarks: Clarithromycin prevents bacteria from growing by interfering with their protein synthesis.

MEB1 and Hydrophobic Protein: bacterio-opsin
Column: MEB1-5-100-5
Solutes: 1 = bacterio-opsin
Eluent: 0.02% TFA Water:0.02% TFA CAN, Hold 100:0 (v:v) 5 mins then gradient to 60:40 (v:v) in 10 mins
Flow: 1.0 mL/min
Detector: UV; 220nm
Temp: 30° C
Remarks: Bacterio-opsin is a very hydrophobic membrane protein useful in the study of protein folding, stability and structure.

MEB2 and Biomolecules: Laccase
Column: MEB2-5-300-15
Solutes: 1 = Laccase
2 = unknown
Eluent: 0.02% TFA Water:0.02% TFA ACN, Hold 100:0 (v:v) 5 mins then gradient to 60:40 (v:v) in 10 mins
Flow: 1.0 mL/min
Detector: UV; 215nm
Temp: 30° C
Remarks: Laccases are copper-containing oxidase enzymes that are found in many plants, fungi, and microorganisms

MEB2 and Drugs: Zantac®
Column: MEB2-5-100-15
Solutes: 1 = ranitidine HCL
Eluent: 0.02% TFA Water:0.02% TFA CAN, Hold 100:0 (v:v) 5 mins then gradient to 60:40 (v:v) in 10 mins
Flow: 1.0 mL/min
Detector: UV; 315nm
Temp: 30° C
Remarks: Ranitidine (zantac) is used in treatment of peptic ulcer and gastroesophageal reflux as well as treatment of skin conditions such as hives

MEB4 and Hydrophobic Protein: Collagen
Column: MEB4-5-300-15
Solutes: 1 = collagen
Eluent: A=0.05% TFA /water B=0.05%% TFA/ACN;
A:B (20:80) to A:B (80:20) in 10 min
Flow: 1.0 mL/min
Detector: UV; 320nm
Temp: 30° C
Remarks: Virtually no nonspecific adsorption of proteins

MEB4 and Protein molecules: Insulin variants
Column: MEB4-5-300-25
Solutes: 1 = Chicken Insulin
2 = Bovine Insulin
3 = Rabbit Insulin
4 = Human Insulin
5 = Porcine Insulin
Eluent: A: 0.05% TFA-water B=0.05% TFA-ACN
20-40% B in 20 min
Flow: 1.0 mL/min
Detector: UV; 220nm
Temp: 30° C
Remarks: Virtually no nonspecific adsorption of proteins

Specifications:  Columns, SMT MEB (Methyl, Ethyl and Butyl) C1, C2 and C4 Short Carbon Chain HPLC

NumberDescriptionPrice $
MEB1-5-100-2 SMT MEB1 C1 Column, 5 µm, 100Å, 20 mm L x 4.6 mm I.D498.15
MEB1-5-100-3 SMT MEB1 C1 Column, 5 µm, 100Å, 30 mm L x 4.6 mm I.D511.65
MEB1-5-100-5 SMT MEB1 C1 Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D525.15
MEB1-5-100-7.5 SMT MEB1 C1 Column, 5 µm, 100Å, 75 mm L x 4.6 mm I.D565.65
MEB1-5-100-10 SMT MEB1 C1 Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D592.65
MEB1-5-100-15 SMT MEB1 C1 Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D660.15
MEB1-5-100-25 SMT MEB1 C1 Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D700.65
MEB1-5-100-30 SMT MEB1 C1 Column, 5 µm, 100Å, 300 mm L x 4.6 mm I.D795.15
MEB1-5-100-34 SMT MEB1 C1 Column, 5 µm, 100Å, 30 mm L x 3.9 mm I.D498.15
MEB1-5-100-34 SMT MEB1 C1 Column, 5 µm, 100Å, 30 mm L x 3.9 mm I.D511.65
MEB1-5-100-54 SMT MEB1 C1 Column, 5 µm, 100Å, 50 mm L x 3.9 mm I.D525.15
MEB1-5-100-7.54 SMT MEB1 C1 Column, 5 µm, 100Å, 75 mm L x 3.9 mm I.D565.65
MEB1-5-100-104 SMT MEB1 C1 Column, 5 µm, 100Å, 100 mm L x 3.9 mm I.D592.65
MEB1-5-100-154 SMT MEB1 C1 Column, 5 µm, 100Å, 150 mm L x 3.9 mm I.D660.15
MEB1-5-100-254 SMT MEB1 C1 Column, 5 µm, 100Å, 250 mm L x 3.9 mm I.D700.65
MEB1-5-100-304 SMT MEB1 C1 Column, 5 µm, 100Å, 300 mm L x 3.9 mm I.D795.15
MEB1-5-300-2 SMT MEB1 C1 Column, 5 µm, 300Å, 20 mm L x 4.6 mm I.D369.00
MEB1-5-300-3 SMT MEB1 C1 Column, 5 µm, 300Å, 30 mm L x 4.6 mm I.D379.00
MEB1-5-300-5 SMT MEB1 C1 Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D389.00
MEB1-5-300-7.5 SMT MEB1 C1 Column, 5 µm, 300Å, 75 mm L x 4.6 mm I.D419.00
MEB1-5-300-10 SMT MEB1 C1 Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D439.00
MEB1-5-300-15 SMT MEB1 C1 Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D489.00
MEB1-5-300-25 SMT MEB1 C1 Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D519.00
MEB1-5-300-30 SMT MEB1 C1 Column, 5 µm, 300Å, 300 mm L x 4.6 mm I.D589.00
MEB1-5-300-2 SMT MEB1 C1 Column, 5 µm, 300Å, 20 mm L x 3.9 mm I.D369.00
MEB1-5-300-3 SMT MEB1 C1 Column, 5 µm, 300Å, 30 mm L x 3.9 mm I.D379.00
MEB1-5-300-54 SMT MEB1 C1 Column, 5 µm, 300Å, 50 mm L x 3.9 mm I.D389.00
MEB1-5-300-7.54 SMT MEB1 C1 Column, 5 µm, 300Å, 75 mm L x 3.9 mm I.D419.00
MEB1-5-300-104 SMT MEB1 C1 Column, 5 µm, 300Å, 100 mm L x 3.9 mm I.D439.00
MEB1-5-300-154 SMT MEB1 C1 Column, 5 µm, 300Å, 150 mm L x 3.9 mm I.D489.00
MEB1-5-300-254 SMT MEB1 C1 Column, 5 µm, 300Å, 250 mm L x 3.9 mm I.D519.00
MEB1-5-300-304 SMT MEB1 C1 Column, 5 µm, 300Å, 300 mm L x 3.9 mm I.D589.00
MEB2-5-100-2 SMT MEB2 C2 Column, 5 µm, 100Å, 20 mm L x 4.6 mm I.D498.15
MEB2-5-100-3 SMT MEB2 C2 Column, 5 µm, 100Å, 30 mm L x 4.6 mm I.D511.65
MEB2-5-100-5 SMT MEB2 C2 Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D525.15
MEB2-5-100-7.5 SMT MEB2 C2 Column, 5 µm, 100Å, 75 mm L x 4.6 mm I.D565.65
MEB2-5-100-10 SMT MEB2 C2 Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D592.65
MEB2-5-100-15 SMT MEB2 C2 Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D660.15
MEB2-5-100-25 SMT MEB2 C2 Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D700.65
MEB2-5-100-30 SMT MEB2 C2 Column, 5 µm, 100Å, 300 mm L x 4.6 mm I.D795.15
MEB2-5-100-24 SMT MEB2 C2 Column, 5 µm, 100Å, 20 mm L x 3.9 mm I.D498.15
MEB2-5-100-34 SMT MEB2 C2 Column, 5 µm, 100Å, 30 mm L x 3.9 mm I.D511.65
MEB2-5-100-54 SMT MEB2 C2 Column, 5 µm, 100Å, 50 mm L x 3.9 mm I.D525.15
MEB2-5-100-7.54 SMT MEB2 C2 Column, 5 µm, 100Å, 75 mm L x 3.9 mm I.D565.65
MEB2-5-100-104 SMT MEB2 C2 Column, 5 µm, 100Å, 100 mm L x 3.9 mm I.D592.65
MEB2-5-100-154 SMT MEB2 C2 Column, 5 µm, 100Å, 150 mm L x 3.9 mm I.D660.15
MEB2-5-100-254 SMT MEB2 C2 Column, 5 µm, 100Å, 250 mm L x 3.9 mm I.D700.65
MEB2-5-100-304 SMT MEB2 C2 Column, 5 µm, 100Å, 300 mm L x 3.9 mm I.D795.15
MEB2-5-300-2 SMT MEB2 C2 Column, 5 µm, 300Å, 20 mm L x 4.6 mm I.D498.15
MEB2-5-300-3 SMT MEB2 C2 Column, 5 µm, 300Å, 30 mm L x 4.6 mm I.D511.65
MEB2-5-300-5 SMT MEB2 C2 Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D525.15
MEB2-5-300-7.5 SMT MEB2 C2 Column, 5 µm, 300Å, 75 mm L x 4.6 mm I.D565.65
MEB2-5-300-10 SMT MEB2 C2 Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D592.65
MEB2-5-300-15 SMT MEB2 C2 Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D660.15
MEB2-5-300-25 SMT MEB2 C2 Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D700.65
MEB2-5-300-30 SMT MEB2 C2 Column, 5 µm, 300Å, 300 mm L x 4.6 mm I.D795.15
MEB2-5-300-34 SMT MEB2 C2 Column, 5 µm, 300Å, 30 mm L x 3.9 mm I.D498.15
MEB2-5-300-54 SMT MEB2 C2 Column, 5 µm, 300Å, 50 mm L x 3.9 mm I.D511.65
MEB2-5-300-7.54 SMT MEB2 C2 Column, 5 µm, 300Å, 75 mm L x 3.9 mm I.D525.15
MEB2-5-300-104 SMT MEB2 C2 Column, 5 µm, 300Å, 100 mm L x 3.9 mm I.D565.65
MEB2-5-300-154 SMT MEB2 C2 Column, 5 µm, 300Å, 150 mm L x 3.9 mm I.D592.65
MEB2-5-300-254 SMT MEB2 C2 Column, 5 µm, 300Å, 250 mm L x 3.9 mm I.D660.15
MEB2-5-300-304 SMT MEB2 C2 Column, 5 µm, 300Å, 300 mm L x 3.9 mm I.D700.65
MEB4-5-100-2 SMT MEB4 C4 Column, 5 µm, 100Å, 20 mm L x 4.6 mm I.D498.15
MEB4-5-100-3 SMT MEB4 C4 Column, 5 µm, 100Å, 30 mm L x 4.6 mm I.D511.65
MEB4-5-100-5 SMT MEB4 C4 Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D525.15
MEB4-5-100-7.5 SMT MEB4 C4 Column, 5 µm, 100Å, 75 mm L x 4.6 mm I.D565.65
MEB4-5-100-10 SMT MEB4 C4 Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D592.65
MEB4-5-100-15 SMT MEB4 C4 Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D660.15
MEB4-5-100-25 SMT MEB4 C4 Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D700.65
MEB4-5-100-30 SMT MEB4 C4 Column, 5 µm, 100Å, 300 mm L x 4.6 mm I.D795.15
MEB4-5-100-24 SMT MEB4 C4 Column, 5 µm, 100Å, 20 mm L x 3.9 mm I.D498.15
MEB4-5-100-34 SMT MEB4 C4 Column, 5 µm, 100Å, 30 mm L x 3.9 mm I.D511.65
MEB4-5-100-54 SMT MEB4 C4 Column, 5 µm, 100Å, 50 mm L x 3.9 mm I.D525.15
MEB4-5-100-7.54 SMT MEB4 C4 Column, 5 µm, 100Å, 75 mm L x 3.9 mm I.D565.65
MEB4-5-100-104 SMT MEB4 C4 Column, 5 µm, 100Å, 100 mm L x 3.9 mm I.D592.65
MEB4-5-100-154 SMT MEB4 C4 Column, 5 µm, 100Å, 150 mm L x 3.9 mm I.D660.15
MEB4-5-100-254 SMT MEB4 C4 Column, 5 µm, 100Å, 250 mm L x 3.9 mm I.D700.65
MEB4-5-100-304 SMT MEB4 C4 Column, 5 µm, 100Å, 300 mm L x 3.9 mm I.D795.15
MEB4-5-300-2 SMT MEB4 C4 Column, 5 µm, 300Å, 20 mm L x 4.6 mm I.D498.15
MEB4-5-300-3 SMT MEB4 C4 Column, 5 µm, 300Å, 30 mm L x 4.6 mm I.D511.65
MEB4-5-300-5 SMT MEB4 C4 Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D525.15
MEB4-5-300-7.5 SMT MEB4 C4 Column, 5 µm, 300Å, 75 mm L x 4.6 mm I.D565.65
MEB4-5-300-10 SMT MEB4 C4 Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D592.65
MEB4-5-300-15 SMT MEB4 C4 Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D660.15
MEB4-5-300-25 SMT MEB4 C4 Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D700.65
MEB4-5-300-30 SMT MEB4 C4 Column, 5 µm, 300Å, 300 mm L x 4.6 mm I.D795.15
MEB4-5-300-24 SMT MEB4 C4 Column, 5 µm, 300Å, 20 mm L x 3.9 mm I.D498.15
MEB4-5-300-34 SMT MEB4 C4 Column, 5 µm, 300Å, 30 mm L x 3.9 mm I.D511.65
MEB4-5-300-54 SMT MEB4 C4 Column, 5 µm, 300Å, 50 mm L x 3.9 mm I.D525.15
MEB4-5-300-7.54 SMT MEB4 C4 Column, 5 µm, 300Å, 75 mm L x 3.9 mm I.D565.65
MEB4-5-300-104 SMT MEB4 C4 Column, 5 µm, 300Å, 100 mm L x 3.9 mm I.D592.65
MEB4-5-300-154 SMT MEB4 C4 Column, 5 µm, 300Å, 150 mm L x 3.9 mm I.D660.15
MEB4-5-300-254 SMT MEB4 C4 Column, 5 µm, 300Å, 250 mm L x 3.9 mm I.D700.65
MEB4-5-300-304 SMT MEB4 C4 Column, 5 µm, 300Å, 300 mm L x 3.9 mm I.D795.15